Real-time in vivo imaging of extracellular ATP in the brain with a hybrid-type fluorescent sensor

Adenosine 5’ triphosphate (ATP) is a ubiquitous extracellular signaling messenger. Here, we describe a method for in-vivo imaging of extracellular ATP with high spatiotemporal resolution. We prepared a comprehensive set of cysteine-substitution mutants of ATP-binding protein, Bacillus FoF(1)-ATP syn...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Kitajima, Nami, Takikawa, Kenji, Sekiya, Hiroshi, Satoh, Kaname, Asanuma, Daisuke, Sakamoto, Hirokazu, Takahashi, Shodai, Hanaoka, Kenjiro, Urano, Yasuteru, Namiki, Shigeyuki, Iino, Masamitsu, Hirose, Kenzo
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2020
Assuntos:
Biochemistry and Chemical Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7398694/
https://ncbi.nlm.nih.gov/pubmed/32648544
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.57544
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