Unlabeled lysophosphatidic acid receptor binding in free solution as determined by a compensated interferometric reader

Native interactions between lysophospholipids (LPs) and their cognate LP receptors are difficult to measure because of lipophilicity and/or the adhesive properties of lipids, which contribute to high levels of nonspecific binding in cell membrane preparations. Here, we report development of a free-s...

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Bibliografske podrobnosti
izdano v:J Lipid Res
Main Authors: Ray, Manisha, Nagai, Kazufumi, Kihara, Yasuyuki, Kussrow, Amanda, Kammer, Michael N., Frantz, Aaron, Bornhop, Darryl J., Chun, Jerold
Format: Artigo
Jezik:Inglês
Izdano: The American Society for Biochemistry and Molecular Biology 2020
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Methods
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7397748/
https://ncbi.nlm.nih.gov/pubmed/32513900
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D120000880
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