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Engineering of a Red Fluorogenic Protein/Merocyanine Complex for Live Cell Imaging

A reengineered human Cellular Retinol Binding Protein II (hCRBPII), a 15 kDa protein belonging to the intracellular Lipid Binding Protein (iLBP) family, generates a highly fluorescent red pigment through the covalent linkage of a merocyanine aldehyde to an active site Lys residue. The complex exhibi...

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Bibliografiske detaljer
Udgivet i:Chembiochem
Main Authors: Santos, Elizabeth M., Berbasova, Tetyana, Wang, Wenjing, Salmani, Rahele Esmatpour, Sheng, Wei, Vasileiou, Chrysoula, Geiger, James H., Borhan, Babak
Format: Artigo
Sprog:Inglês
Udgivet: 2019
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7379159/
https://ncbi.nlm.nih.gov/pubmed/31482666
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201900428
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