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Quantifying mRNA levels across tissue sections with 2D-RT-qPCR

Measurement of mRNA levels across tissue samples facilitates an understanding of how genes function and what their roles are in disease. Quantifying low-abundance mRNA requires a workflow that preserves spatial information, isolates RNA, and performs reverse-transcriptase quantitative polymerase cha...

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Détails bibliographiques
Publié dans:Anal Bioanal Chem
Auteurs principaux: Armani, Michael, Tangrea, Michael A., Shapiro, Benjamin, Emmert-Buck, Michael R., Smela, Elisabeth
Format: Artigo
Langue:Inglês
Publié: 2011
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7375691/
https://ncbi.nlm.nih.gov/pubmed/21559756
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00216-011-5062-8
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