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Improved bacterial RNA-seq by Cas9-based depletion of ribosomal RNA reads
A major challenge for RNA-seq analysis of gene expression is to achieve sufficient coverage of informative nonribosomal transcripts. In eukaryotic samples, this is typically achieved by selective oligo(dT)-priming of messenger RNAs to exclude ribosomal RNA (rRNA) during cDNA synthesis. However, this...
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| 發表在: | RNA |
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| Main Authors: | , , , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Cold Spring Harbor Laboratory Press
2020
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7373992/ https://ncbi.nlm.nih.gov/pubmed/32345633 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.075945.120 |
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