Improved bacterial RNA-seq by Cas9-based depletion of ribosomal RNA reads

A major challenge for RNA-seq analysis of gene expression is to achieve sufficient coverage of informative nonribosomal transcripts. In eukaryotic samples, this is typically achieved by selective oligo(dT)-priming of messenger RNAs to exclude ribosomal RNA (rRNA) during cDNA synthesis. However, this...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:RNA
Hlavní autoři: Prezza, Gianluca, Heckel, Tobias, Dietrich, Sascha, Homberger, Christina, Westermann, Alexander J., Vogel, Jörg
Médium: Artigo
Jazyk:Inglês
Vydáno: Cold Spring Harbor Laboratory Press 2020
Témata:
Method
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7373992/
https://ncbi.nlm.nih.gov/pubmed/32345633
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.075945.120
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo otaguje tento záznam!

Podobné jednotky