In Situ Detection of Endogenous HIV Activation by Dynamic Nuclear Polarization NMR and Flow Cytometry

We demonstrate for the first time in-cell dynamic nuclear polarization (DNP) in conjunction with flow cytometry sorting to address the cellular heterogeneity of in-cell samples. Utilizing a green fluorescent protein (GFP) reporter of HIV reactivation, we correlate increased (15)N resonance intensity...

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Vydáno v:Int J Mol Sci
Hlavní autoři: Overall, Sarah A., Price, Lauren E., Albert, Brice J., Gao, Chukun, Alaniva, Nicholas, Judge, Patrick T., Sesti, Erika L., Wender, Paul A., Kyei, George B., Barnes, Alexander B.
Médium: Artigo
Jazyk:Inglês
Vydáno: MDPI 2020
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7369949/
https://ncbi.nlm.nih.gov/pubmed/32629894
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms21134649
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