Prediction-based highly sensitive CRISPR off-target validation using target-specific DNA enrichment

CRISPR effectors, which comprise a CRISPR-Cas protein and a guide (g)RNA derived from the bacterial immune system, are widely used for target-specific genome editing. When the gRNA recognizes genomic loci with sequences that are similar to the target, deleterious mutations can occur. Off-target muta...

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Pubblicato in:Nat Commun
Autori principali: Kang, Seung-Hun, Lee, Wi-jae, An, Ju-Hyun, Lee, Jong-Hee, Kim, Young-Hyun, Kim, Hanseop, Oh, Yeounsun, Park, Young-Ho, Jin, Yeung Bae, Jun, Bong-Hyun, Hur, Junho K., Kim, Sun-Uk, Lee, Seung Hwan
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group UK 2020
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7368065/
https://ncbi.nlm.nih.gov/pubmed/32681048
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-17418-8
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