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MS2-TRIBE Evaluates Both Protein-RNA Interactions and Nuclear Organization of Transcription by RNA Editing

Both UV-cross-linking and immunoprecipitation (CLIP) and RNA editing (TRIBE) can identify the targets of RNA-binding proteins. To evaluate false-positives of CLIP and TRIBE, endogenous β-actin mRNA was tagged with MS2 stem loops, making it the only bona fide target mRNA for the MS2 capsid protein (M...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:iScience
Hauptverfasser: Biswas, Jeetayu, Rahman, Reazur, Gupta, Varun, Rosbash, Michael, Singer, Robert H.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier 2020
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7363692/
https://ncbi.nlm.nih.gov/pubmed/32674054
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.isci.2020.101318
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