Structure-based design of gRNA for Cas13

Cas13 endonuclease activity depends on the RNA local secondary structure with strong preference for single-stranded (SS) regions. Hence, it becomes indispensable to identify the SS regions for effective Cas13 mediated RNA knockdown. We herein present rational gRNA design by integrating experimental...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Bandaru, Srinivas, Tsuji, Mika Higashide, Shimizu, Yurika, Usami, Kaya, Lee, Suni, Takei, Naoko Kumagai, Yoshitome, Kei, Nishimura, Yasumitsu, Otsuki, Takemi, Ito, Tatsuo
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2020
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7360764/
https://ncbi.nlm.nih.gov/pubmed/32665590
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-68459-4
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