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The Influence of DNA Extraction and Lipid Removal on Human Milk Bacterial Profiles

Culture-independent molecular techniques have advanced the characterization of environmental and human samples including the human milk (HM) bacteriome. However, extraction of high-quality genomic DNA that is representative of the bacterial population in samples is crucial. Lipids removal from HM pr...

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Detalles Bibliográficos
Publicado en:Methods Protoc
Main Authors: Ojo-Okunola, Anna, Claassen-Weitz, Shantelle, Mwaikono, Kilaza S., Gardner-Lubbe, Sugnet, Zar, Heather J., Nicol, Mark P., du Toit, Elloise
Formato: Artigo
Idioma:Inglês
Publicado: MDPI 2020
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7359716/
https://ncbi.nlm.nih.gov/pubmed/32429170
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/mps3020039
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