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Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens
BACKGROUND: Careful consideration of experimental artefacts is required in order to successfully apply high-throughput 16S ribosomal ribonucleic acid (rRNA) gene sequencing technology. Here we introduce experimental design, quality control and “denoising” approaches for sequencing low biomass specim...
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| Publicado en: | BMC Microbiol |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
BioMed Central
2020
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7218582/ https://ncbi.nlm.nih.gov/pubmed/32397992 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12866-020-01795-7 |
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