Llwytho...

Structure-guided engineering of the homodimeric Mango-IV fluorescence turn-on aptamer yields an RNA FRET pair

Fluorescent RNA aptamers have been used in cells as biosensor reporters and tags for tracking transcripts. Recently, combined SELEX and microfluidic fluorescence sorting yielded three aptamers that activate fluorescence of TO1-Biotin: Mango-II, Mango-III, and Mango-IV. Of these, Mango-IV was best at...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Structure
Prif Awduron: Trachman, Robert J., Cojocaru, Razvan, Wu, Di, Piszczek, Grzegorz, Ryckelynck, Michael, Unrau, Peter J., Ferré-D’Amaré, Adrian R.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2020
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7347457/
https://ncbi.nlm.nih.gov/pubmed/32386573
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.str.2020.04.007
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