Structure-guided engineering of the homodimeric Mango-IV fluorescence turn-on aptamer yields an RNA FRET pair

Fluorescent RNA aptamers have been used in cells as biosensor reporters and tags for tracking transcripts. Recently, combined SELEX and microfluidic fluorescence sorting yielded three aptamers that activate fluorescence of TO1-Biotin: Mango-II, Mango-III, and Mango-IV. Of these, Mango-IV was best at...

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Enregistré dans:
Détails bibliographiques
Publié dans:Structure
Auteurs principaux: Trachman, Robert J., Cojocaru, Razvan, Wu, Di, Piszczek, Grzegorz, Ryckelynck, Michael, Unrau, Peter J., Ferré-D’Amaré, Adrian R.
Format: Artigo
Langue:Inglês
Publié: 2020
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Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7347457/
https://ncbi.nlm.nih.gov/pubmed/32386573
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.str.2020.04.007
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