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Two particle-picking procedures for filamentous proteins: SPHIRE-crYOLO filament mode and SPHIRE-STRIPER

Structure determination of filamentous molecular complexes involves the selection of filaments from cryo-EM micrographs. The automatic selection of helical specimens is particularly difficult, and thus many challenging samples with issues such as contamination or aggregation are still manually picke...

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Detalhes bibliográficos
Publicado no:Acta Crystallogr D Struct Biol
Main Authors: Wagner, Thorsten, Lusnig, Luca, Pospich, Sabrina, Stabrin, Markus, Schönfeld, Fabian, Raunser, Stefan
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7336381/
https://ncbi.nlm.nih.gov/pubmed/32627734
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2059798320007342
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