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Purification of Tag-Free Chlamydia trachomatis Scc4 for Structural Studies Using Sarkosyl-Assisted on-Column Complex Dissociation

Chlamydia trachomatis is an obligate intracellular bacterial pathogen that causes the most common sexually transmitted bacterial disease in the world. The bacterium has a unique biphasic developmental cycle with a type III secretion system (T3SS) to invade host cells. Scc4 is a class I T3SS chaperon...

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Detalhes bibliográficos
Publicado no:Biochemistry
Main Authors: Ukwaththage, Thilini O., Goodwin, Octavia Y., Songok, Abigael C., Tafaro, Alexa M., Shen, Li, Macnaughtan, Megan A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7332156/
https://ncbi.nlm.nih.gov/pubmed/31545893
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.9b00665
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