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Analysis of the influence of flame sterilization included in sampling operations on shake-flask cultures of microorganisms

Shake-flask cultures of microorganisms involve flame sterilization during sampling, which produces combustion gas with high CO(2) concentrations. The gaseous destination has not been deeply analyzed. Our aim was to investigate the effect of flame sterilization on the headspace of the flask and on th...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Takahashi, Masato, Honzawa, Takafumi, Tominaga, Ryuichi, Aoyagi, Hideki
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7326993/
https://ncbi.nlm.nih.gov/pubmed/32606322
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-66810-3
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