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An in vitro Assay to Screen for Substrates of PKA Using Phospho-motif-specific Antibodies
Kinases function as regulators of many cellular processes such as cell migration. These enzymes typically phosphorylate target motif sequences. Mass spec or phospho-specific antibody detection can be used to determine whether a kinase can phosphorylate proteins of interest, however, mass spec can be...
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| Publicado no: | Bio Protoc |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Bio-Protocol
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7324021/ https://ncbi.nlm.nih.gov/pubmed/32601599 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3587 |
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