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qPCR multiplex detection of microRNA and messenger RNA in a single reaction

Reverse Transcription-Quantitative PCR (RT-qPCR) is one of the standards for analytical measurement of different RNA species in biological models. However, current Reverse Transcription (RT) based priming strategies are unable to synthesize differing RNAs and ncRNAs especially miRNAs, within a singl...

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Publicat a:PeerJ
Autors principals: Khoury, Samantha, Tran, Nham
Format: Artigo
Idioma:Inglês
Publicat: PeerJ Inc. 2020
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7321665/
https://ncbi.nlm.nih.gov/pubmed/32617186
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7717/peerj.9004
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