Erythropoietin production by the kidney and the liver in response to severe hypoxia evaluated by Western blotting with deglycosylation

The detection of erythropoietin (Epo) protein by Western blotting has required pre‐purification of the sample. We developed a new Western blot method to detect plasma and urinary Epo using deglycosylation. Epo in urine and tissue, and erythropoiesis‐stimulating agents (ESAs) in urine were directly d...

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Dettagli Bibliografici
Pubblicato in:Physiol Rep
Autori principali: Yasuoka, Yukiko, Fukuyama, Takashi, Izumi, Yuichiro, Nakayama, Yushi, Inoue, Hideki, Yanagita, Kengo, Oshima, Tomomi, Yamazaki, Taiga, Uematsu, Takayuki, Kobayashi, Noritada, Shimada, Yoshitaka, Nagaba, Yasushi, Mukoyama, Masashi, Yamashita, Tetsuro, Sato, Yuichi, Sands, Jeff M., Kawahara, Katsumasa, Nonoguchi, Hiroshi
Natura: Artigo
Lingua:Inglês
Pubblicazione: John Wiley and Sons Inc. 2020
Soggetti:
Original Research
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7319944/
https://ncbi.nlm.nih.gov/pubmed/32592328
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.14814/phy2.14485
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