Erythropoietin production by the kidney and the liver in response to severe hypoxia evaluated by Western blotting with deglycosylation

The detection of erythropoietin (Epo) protein by Western blotting has required pre‐purification of the sample. We developed a new Western blot method to detect plasma and urinary Epo using deglycosylation. Epo in urine and tissue, and erythropoiesis‐stimulating agents (ESAs) in urine were directly d...

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Detalhes bibliográficos
Publicado no:Physiol Rep
Main Authors: Yasuoka, Yukiko, Fukuyama, Takashi, Izumi, Yuichiro, Nakayama, Yushi, Inoue, Hideki, Yanagita, Kengo, Oshima, Tomomi, Yamazaki, Taiga, Uematsu, Takayuki, Kobayashi, Noritada, Shimada, Yoshitaka, Nagaba, Yasushi, Mukoyama, Masashi, Yamashita, Tetsuro, Sato, Yuichi, Sands, Jeff M., Kawahara, Katsumasa, Nonoguchi, Hiroshi
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2020
Assuntos:
Original Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7319944/
https://ncbi.nlm.nih.gov/pubmed/32592328
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.14814/phy2.14485
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