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Fluorescently Labelled ATP Analogues for Direct Monitoring of Ubiquitin Activation

Simple and robust assays to monitor enzymatic ATP cleavage with high efficiency in real‐time are scarce. To address this shortcoming, we developed fluorescently labelled adenosine tri‐, tetra‐ and pentaphosphate analogues of ATP. The novel ATP analogues bear — in contrast to earlier reports — only a...

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Detalles Bibliográficos
Publicado en:Chemistry
Main Authors: Hammler, Daniel, Stuber, Katrin, Offensperger, Fabian, Scheffner, Martin, Zumbusch, Andreas, Marx, Andreas
Formato: Artigo
Idioma:Inglês
Publicado: John Wiley and Sons Inc. 2020
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7317923/
https://ncbi.nlm.nih.gov/pubmed/32154932
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/chem.202001091
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