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Fluorescently Labelled ATP Analogues for Direct Monitoring of Ubiquitin Activation

Simple and robust assays to monitor enzymatic ATP cleavage with high efficiency in real‐time are scarce. To address this shortcoming, we developed fluorescently labelled adenosine tri‐, tetra‐ and pentaphosphate analogues of ATP. The novel ATP analogues bear — in contrast to earlier reports — only a...

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Détails bibliographiques
Publié dans:Chemistry
Auteurs principaux: Hammler, Daniel, Stuber, Katrin, Offensperger, Fabian, Scheffner, Martin, Zumbusch, Andreas, Marx, Andreas
Format: Artigo
Langue:Inglês
Publié: John Wiley and Sons Inc. 2020
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7317923/
https://ncbi.nlm.nih.gov/pubmed/32154932
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/chem.202001091
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