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Fluorescently Labelled ATP Analogues for Direct Monitoring of Ubiquitin Activation

Simple and robust assays to monitor enzymatic ATP cleavage with high efficiency in real‐time are scarce. To address this shortcoming, we developed fluorescently labelled adenosine tri‐, tetra‐ and pentaphosphate analogues of ATP. The novel ATP analogues bear — in contrast to earlier reports — only a...

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Detalhes bibliográficos
Publicado no:Chemistry
Main Authors: Hammler, Daniel, Stuber, Katrin, Offensperger, Fabian, Scheffner, Martin, Zumbusch, Andreas, Marx, Andreas
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7317923/
https://ncbi.nlm.nih.gov/pubmed/32154932
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/chem.202001091
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