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TET1 Deficiency Impairs Morphogen-free Differentiation of Human Embryonic Stem Cells to Neuroectoderm

The TET family of 5-methylcytosine (5mC) dioxygenases plays critical roles in development by modifying DNA methylation. Using CRISPR, we inactivated the TET1 gene in H9 human embryonic stem cells (hESCs). Mutant H9 hESCs remained pluripotent, even though the level of hydroxymethylcytosine (5hmC) dec...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Li, Hanqin, Hu, Zhixing, Jiang, Houbo, Pu, Jiali, Selli, Ilana, Qiu, Jingxin, Zhang, Baorong, Feng, Jian
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7316867/
https://ncbi.nlm.nih.gov/pubmed/32587369
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-67143-x
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