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Successful delivery of large-size CRISPR/Cas9 vectors in hard-to-transfect human cells using small plasmids

With the rise of new powerful genome engineering technologies, such as CRISPR/Cas9, cell models can be engineered effectively to accelerate basic and disease research. The most critical step in this procedure is the efficient delivery of foreign nucleic acids into cells by cellular transfection. Sin...

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書目詳細資料
發表在:Commun Biol
Main Authors: Søndergaard, Jonas Nørskov, Geng, Keyi, Sommerauer, Christian, Atanasoai, Ionut, Yin, Xiushan, Kutter, Claudia
格式: Artigo
語言:Inglês
出版: Nature Publishing Group UK 2020
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC7305135/
https://ncbi.nlm.nih.gov/pubmed/32561814
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s42003-020-1045-7
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