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Successful delivery of large-size CRISPR/Cas9 vectors in hard-to-transfect human cells using small plasmids

With the rise of new powerful genome engineering technologies, such as CRISPR/Cas9, cell models can be engineered effectively to accelerate basic and disease research. The most critical step in this procedure is the efficient delivery of foreign nucleic acids into cells by cellular transfection. Sin...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Commun Biol
Main Authors: Søndergaard, Jonas Nørskov, Geng, Keyi, Sommerauer, Christian, Atanasoai, Ionut, Yin, Xiushan, Kutter, Claudia
Format: Artigo
Jezik:Inglês
Izdano: Nature Publishing Group UK 2020
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7305135/
https://ncbi.nlm.nih.gov/pubmed/32561814
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s42003-020-1045-7
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