CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation

The CRISPR (clustered regularly interspaced short palindromic repeats)–Cas (CRISPR-associated) nuclease system represents an efficient tool for genome editing and gene function analysis. It consists of two components: single-guide RNA (sgRNA) and the enzyme Cas9. Typical sgRNA and Cas9 intracellular...

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Detalhes bibliográficos
Publicado no:Sci Adv
Main Authors: Han, Xin, Liu, Zongbin, Jo, Myeong chan, Zhang, Kai, Li, Ying, Zeng, Zihua, Li, Nan, Zu, Youli, Qin, Lidong
Formato: Artigo
Idioma:Inglês
Publicado em: American Association for the Advancement of Science 2015
Assuntos:
Research Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4643799/
https://ncbi.nlm.nih.gov/pubmed/26601238
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/sciadv.1500454
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