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Constructing arrays of nucleosome positioning sequences using Gibson Assembly for single-molecule studies

As the basic building blocks of chromatin, nucleosomes play a key role in dictating the accessibility of the eukaryotic genome. Consequently, nucleosomes are involved in essential genomic transactions such as DNA transcription, replication and repair. In order to unravel the mechanisms by which nucl...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Spakman, Dian, King, Graeme A., Peterman, Erwin J. G., Wuite, Gijs J. L.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7303147/
https://ncbi.nlm.nih.gov/pubmed/32555215
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-66259-4
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