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Designing sgRNAs for CRISPR-BEST base editing applications with CRISPy-web 2.0

CRISPR/Cas9 systems are an established tool in genome engineering. As double strand breaks caused by the standard Cas9-based knock-out techniques can be problematic in some organisms, new systems were developed that can efficiently create knock-outs without causing double strand breaks to elegantly...

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Detalhes bibliográficos
Publicado no:Synth Syst Biotechnol
Main Authors: Blin, Kai, Shaw, Simon, Tong, Yaojun, Weber, Tilmann
Formato: Artigo
Idioma:Inglês
Publicado em: KeAi Publishing 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7301206/
https://ncbi.nlm.nih.gov/pubmed/32596519
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.synbio.2020.05.005
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