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Reductive Amination of α-Ketoglutarate in Metabolite Extracts Results in Glutamate Overestimation

Artifacts due to metabolite extraction, derivatization, and detection techniques can result in aberrant observations that are not accurate representations of actual cell metabolism. Here, we show that α-ketoglutarate (α-KG) is reductively aminated to glutamate in methanol:water metabolite extracts,...

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Bibliografski detalji
Izdano u:J Chromatogr A
Glavni autori: Wiese, Elizabeth K., Hitosugi, Sadae, Buhrow, Sarah A., Loa, Sharon T., Sreedhar, Annapoorna, Reid, Joel M., Gonsalves, Wilson I., Hitosugi, Taro
Format: Artigo
Jezik:Inglês
Izdano: 2020
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7295581/
https://ncbi.nlm.nih.gov/pubmed/32376016
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chroma.2020.461169
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