Anoxic cell rupture of Prevotella bryantii by high-pressure homogenization protects the Na(+)-translocating NADH:quinone oxidoreductase from oxidative damage

Respiratory NADH oxidation in the rumen bacterium Prevotella bryantii is catalyzed by the Na(+)-translocating NADH:quinone oxidoreductase (NQR). A method for cell disruption and membrane isolation of P. bryantii under anoxic conditions using the EmulisFlex-C3 homogenizer is described. We compared NQ...

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Bibliographic Details
Published in:Arch Microbiol
Main Authors: Schleicher, Lena, Fritz, Günter, Seifert, Jana, Steuber, Julia
Format: Artigo
Language:Inglês
Published: Springer Berlin Heidelberg 2020
Subjects:
Short Communication
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7283201/
https://ncbi.nlm.nih.gov/pubmed/31955240
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00203-019-01805-x
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