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Anoxic cell rupture of Prevotella bryantii by high-pressure homogenization protects the Na(+)-translocating NADH:quinone oxidoreductase from oxidative damage
Respiratory NADH oxidation in the rumen bacterium Prevotella bryantii is catalyzed by the Na(+)-translocating NADH:quinone oxidoreductase (NQR). A method for cell disruption and membrane isolation of P. bryantii under anoxic conditions using the EmulisFlex-C3 homogenizer is described. We compared NQ...
Shranjeno v:
| izdano v: | Arch Microbiol |
|---|---|
| Main Authors: | , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Springer Berlin Heidelberg
2020
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7283201/ https://ncbi.nlm.nih.gov/pubmed/31955240 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00203-019-01805-x |
| Oznake: |
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