Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays

The SARS-CoV-2 spike trimer is the primary antigen for several serology assays critical to determining the extent of SARS-CoV-2 exposure in the population. Until stable cell lines are developed to increase the titer of this secreted protein in mammalian cell culture, the low yield of spike protein p...

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Udgivet i:bioRxiv
Main Authors: Esposito, Dominic, Mehalko, Jennifer, Drew, Matthew, Snead, Kelly, Wall, Vanessa, Taylor, Troy, Frank, Peter, Denson, John-Paul, Hong, Min, Gulten, Gulcin, Sadtler, Kaitlyn, Messing, Simon, Gillette, William
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory 2020
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7265690/
https://ncbi.nlm.nih.gov/pubmed/32511418
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/2020.05.27.120204
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