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Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays

The SARS-CoV-2 spike trimer is the primary antigen for several serology assays critical to determining the extent of SARS-CoV-2 exposure in the population. Until stable cell lines are developed to increase the titer of this secreted protein in mammalian cell culture, the low yield of spike protein p...

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Dettagli Bibliografici
Pubblicato in:Protein Expr Purif
Autori principali: Esposito, Dominic, Mehalko, Jennifer, Drew, Matthew, Snead, Kelly, Wall, Vanessa, Taylor, Troy, Frank, Peter, Denson, John-Paul, Hong, Min, Gulten, Gulcin, Sadtler, Kaitlyn, Messing, Simon, Gillette, William
Natura: Artigo
Lingua:Inglês
Pubblicazione: Elsevier Inc. 2020
Soggetti:
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7271859/
https://ncbi.nlm.nih.gov/pubmed/32504802
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2020.105686
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