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Application of poxvirus K3 ortholog as a positive selection marker for constructing recombinant vaccinia viruses with modified host range

Vaccinia virus is capable of replicating in a wide range of vertebrate animal cells. However, deletion of the two virus host range genes, E3L and K3L, would render replication of the virus abortive in all the cell types tested, except the cells with defective PKR and RNase L activity. By expressing...

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Detalhes bibliográficos
Publicado no:MethodsX
Main Authors: Cao, Jingxin, Layne, Christine, Varga, Jessie, Deschambault, Yvon
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7264055/
https://ncbi.nlm.nih.gov/pubmed/32509537
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2020.100918
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