Method: Isolation of Epithelial Cell RNA from Frozen Jejunum Segments While Minimizing Smooth Muscle Cell RNA Contamination

OBJECTIVES: High RNA quality is a prerequisite for accurate PCR and sequencing results. Dissecting a specific tissue fraction from frozen samples while maintaining RNA quality is challenging. Starting with frozen pig jejunum segments, we describe a novel method to isolate epithelial cell RNA while m...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Curr Dev Nutr
Hlavní autoři: Ye, Shumao, Matthan, Nirupa, Lamon-Fava, Stefania, Solano-Aguilar, Gloria, Turner, Jerrold, Wang, Xiang-Dong, Walker, Maura, Chai, Zhi, Lakshman, Sukla, Urban, Joseph, Lichtenstein, Alice
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2020
Témata:
Methods
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7257652/
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/cdn/nzaa056_029
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo otaguje tento záznam!

Podobné jednotky