Method: Isolation of Epithelial Cell RNA from Frozen Jejunum Segments While Minimizing Smooth Muscle Cell RNA Contamination

OBJECTIVES: High RNA quality is a prerequisite for accurate PCR and sequencing results. Dissecting a specific tissue fraction from frozen samples while maintaining RNA quality is challenging. Starting with frozen pig jejunum segments, we describe a novel method to isolate epithelial cell RNA while m...

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Dettagli Bibliografici
Pubblicato in:Curr Dev Nutr
Autori principali: Ye, Shumao, Matthan, Nirupa, Lamon-Fava, Stefania, Solano-Aguilar, Gloria, Turner, Jerrold, Wang, Xiang-Dong, Walker, Maura, Chai, Zhi, Lakshman, Sukla, Urban, Joseph, Lichtenstein, Alice
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2020
Soggetti:
Methods
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7257652/
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/cdn/nzaa056_029
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