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Construction of a Bacillus subtilis and Escherichia coli shuttle vector harboring the fabL gene as a triclosan selection marker
A new plasmid containing a mutated fabL gene from Bacillus subtilis as a triclosan selection marker was developed as a useful B. subtilis/E. coli shuttle vector. The pHT-FabL40 plasmid is stable in both gram-positive and gram-negative hosts with increased plasmid DNA yield in E. coli.
Gardado en:
| Publicado en: | Heliyon |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Elsevier
2020
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7226672/ https://ncbi.nlm.nih.gov/pubmed/32426536 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.heliyon.2020.e03891 |
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