Construction of plasmid cloning vectors for lactic streptococci which also replicate in Bacillus subtilis and Escherichia coli.

The cryptic Streptococcus cremoris Wg2 plasmid pWV01 (1.5 megadaltons) was genetically marked with the chloramphenicol resistance (Cmr) gene from pC194. The recombinant plasmid (pGK1, 2.4 megadaltons) replicated and expressed Cmr in Bacillus subtilis. From this plasmid an insertion-inactivation vect...

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Bibliographic Details
Published in:Appl Environ Microbiol
Main Authors: Kok, J, van der Vossen, J M, Venema, G
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1984
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Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC241602/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6095756/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.48.4.726-731.1984
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