Protease‐resistant streptavidin for interaction proteomics

Streptavidin‐mediated enrichment is a powerful strategy to identify biotinylated biomolecules and their interaction partners; however, intense streptavidin‐derived peptides impede protein identification by mass spectrometry. Here, we present an approach to chemically modify streptavidin, thus render...

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Foilsithe in:Mol Syst Biol
Main Authors: Rafiee, Mahmoud‐Reza, Sigismondo, Gianluca, Kalxdorf, Mathias, Förster, Laura, Brügger, Britta, Béthune, Julien, Krijgsveld, Jeroen
Formáid: Artigo
Teanga:Inglês
Foilsithe: John Wiley and Sons Inc. 2020
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Methods
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7218406/
https://ncbi.nlm.nih.gov/pubmed/32400114
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.15252/msb.20199370
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