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Validation of the Slow Off‐Kinetics of Sirtuin‐Rearranging Ligands (SirReals) by Means of Label‐Free Electrically Switchable Nanolever Technology

We have discovered the sirtuin‐rearranging ligands (SirReals) to be highly potent and selective inhibitors of the NAD(+)‐dependent lysine deacetylase Sirt2. Using a biotinylated SirReal in combination with biolayer interferometry, we previously observed a slow dissociation rate of the inhibitor–enzy...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:Chembiochem
Päätekijät: Schiedel, Matthias, Daub, Herwin, Itzen, Aymelt, Jung, Manfred
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: John Wiley and Sons Inc. 2020
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC7217041/
https://ncbi.nlm.nih.gov/pubmed/31692222
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201900527
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