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Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction

Improper formation or remodeling of the pharyngeal arch arteries (PAAs) 3, 4, and 6 contribute to some of the most severe forms of congenital heart disease. To study the formation of PAAs, we developed a protocol using whole-mount immunofluorescence coupled with benzyl alcohol/benzyl benzoate (BABB)...

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Détails bibliographiques
Publié dans:J Vis Exp
Auteurs principaux: Ramirez, AnnJosette, Astrof, Sophie
Format: Artigo
Langue:Inglês
Publié: 2020
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7216781/
https://ncbi.nlm.nih.gov/pubmed/32310236
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/60797
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