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Use of Phospholipase A(2) for Antigen Retrieval in Zebrafish Whole-Mount Immunohistochemistry

An advantage in zebrafish is that we can identify spatial and temporal patterns of protein expression using whole-mount immunohistochemistry. To allow primary antibodies to interact with their targets, most tissues must undergo some type of antigen retrieval. Many retrieval techniques have utilized...

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Detalhes bibliográficos
Main Authors: Akhtar, Tanveer, Li, Jiannan, Olden, Tasha, Wallace, Kenneth N.
Formato: Artigo
Idioma:Inglês
Publicado em: Mary Ann Liebert, Inc. 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2771340/
https://ncbi.nlm.nih.gov/pubmed/19694523
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/zeb.2009.0588
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