Generation of (34)S-substituted protein-bound [4Fe-4S] clusters using (34)S-L-cysteine

The ability to specifically label the sulphide ions of protein-bound iron–sulphur (FeS) clusters with (34)S isotope greatly facilitates structure–function studies. In particular, it provides insight when using either spectroscopic techniques that probe cluster-associated vibrations, or non-denaturin...

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Bibliographic Details
Published in:Biol Methods Protoc
Main Authors: Crack, Jason C, Stewart, Melissa Y Y, Le Brun, Nick E
Format: Artigo
Language:Inglês
Published: Oxford University Press 2019
Subjects:
Methods Manuscript
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7200944/
https://ncbi.nlm.nih.gov/pubmed/32395620
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/biomethods/bpy015
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