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Rapid identification of human mast cell degranulation regulators using functional genomics coupled to high-resolution confocal microscopy

Targeted functional genomics represents a powerful approach for studying gene function in vivo and in vitro. However, its application to gene expression studies in human mast cells has been hampered by low yields of human mast cell cultures and their poor transfection efficiency. We developed an ima...

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Dades bibliogràfiques
Publicat a:Nat Protoc
Autors principals: Folkerts, Jelle, Gaudenzio, Nicolas, Maurer, Marcus, Hendriks, Rudi W., Stadhouders, Ralph, Tam, See-Ying, Galli, Stephen J.
Format: Artigo
Idioma:Inglês
Publicat: 2020
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7197894/
https://ncbi.nlm.nih.gov/pubmed/32060492
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-019-0288-6
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