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Rapid identification of human mast cell degranulation regulators using functional genomics coupled to high-resolution confocal microscopy

Targeted functional genomics represents a powerful approach for studying gene function in vivo and in vitro. However, its application to gene expression studies in human mast cells has been hampered by low yields of human mast cell cultures and their poor transfection efficiency. We developed an ima...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Folkerts, Jelle, Gaudenzio, Nicolas, Maurer, Marcus, Hendriks, Rudi W., Stadhouders, Ralph, Tam, See-Ying, Galli, Stephen J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7197894/
https://ncbi.nlm.nih.gov/pubmed/32060492
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-019-0288-6
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