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The crystal structure of the naturally split gp41-1 intein guides the engineering of orthogonal split inteins from cis-splicing inteins

Protein trans-splicing catalyzed by split inteins has increasingly become useful as a protein engineering tool. We solved the 1.0 Å-resolution crystal structure of a fused variant from the naturally split gp41–1 intein, previously identified from environmental metagenomic sequence data. The structur...

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Vydáno v:FEBS J
Hlavní autoři: Beyer, Hannes Michael, Mikula, Kornelia Malgorzata, Li, Mi, Wlodawer, Alexander, Iwaï, Hideo
Médium: Artigo
Jazyk:Inglês
Vydáno: 2019
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7190452/
https://ncbi.nlm.nih.gov/pubmed/31665813
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/febs.15113
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