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Synthesis and Bioconjugation of Thiol-Reactive Reagents for the Creation of Site-Selectively Modified Immunoconjugates

Maleimide-bearing bifunctional probes have been employed for decades for the site-selective modification of thiols in biomolecules, especially antibodies. Yet maleimide-based conjugates display limited stability in vivo because the succinimidyl thioether linkage can undergo a retro-Michael reaction....

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Bibliografski detalji
Izdano u:J Vis Exp
Glavni autori: Davydova, Maria, Le Roi, Guillaume Dewaele, Adumeau, Pierre, Zeglis, Brian M.
Format: Artigo
Jezik:Inglês
Izdano: 2019
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7169956/
https://ncbi.nlm.nih.gov/pubmed/30907883
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59063
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