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Nuclease-Assisted Minor Allele Enrichment Using Overlapping Probes-Assisted Amplification-Refractory Mutation System: An Approach for the Improvement of Amplification-Refractory Mutation System-Polymerase Chain Reaction Specificity in Liquid Biopsies

Allele-specific polymerase chain reaction (PCR) (amplification-refractory mutation system, ARMS) is one of the most commonly used methods for mutation detection. However, a main limitation of ARMS-PCR is the false positive results obtained due to nonspecific priming that can take place with wild-typ...

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Detalhes bibliográficos
Publicado no:Anal Chem
Main Authors: Markou, Athina, Tzanikou, Elena, Ladas, Ioannis, Makrigiorgos, G. Mike, Lianidou, Evi
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7154944/
https://ncbi.nlm.nih.gov/pubmed/31538770
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.9b03325
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