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Eliminating effects of particle adsorption to the air/water interface in single-particle cryo-electron microscopy: Bacterial RNA polymerase and CHAPSO

Preferred particle orientation presents a major challenge for many single particle cryo-electron microscopy (cryo-EM) samples. Orientation bias limits the angular information used to generate three-dimensional maps and thus affects the reliability and interpretability of the structural models. The p...

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Detalhes bibliográficos
Publicado no:J Struct Biol X
Main Authors: Chen, James, Noble, Alex J., Kang, Jin Young, Darst, Seth A.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2019
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7153306/
https://ncbi.nlm.nih.gov/pubmed/32285040
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.yjsbx.2019.100005
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