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Targeted nanopore sequencing with Cas9-guided adaptor ligation

Despite recent improvements in sequencing methods, there remains a need for assays that provide high sequencing depth and comprehensive variant detection. Current methods(1-4) are limited by the loss of native modifications, short read length, high input requirements, low yield, or long protocols. H...

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Detalhes bibliográficos
Publicado no:Nat Biotechnol
Main Authors: Gilpatrick, Timothy, Lee, Isac, Graham, James E., Raimondeau, Etienne, Bowen, Rebecca, Heron, Andrew, Downs, Bradley, Sukmar, Saraswati, Sedlazeck, Fritz J, Timp, Winston
Formato: Artigo
Idioma:Inglês
Publicado em: 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7145730/
https://ncbi.nlm.nih.gov/pubmed/32042167
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41587-020-0407-5
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