Increased production of periplasmic proteins in Escherichia coli by directed evolution of the translation initiation region

BACKGROUND: Recombinant proteins are often engineered with an N-terminal signal peptide, which facilitates their secretion to the oxidising environment of the periplasm (gram-negative bacteria) or the culture supernatant (gram-positive bacteria). A commonly encountered problem is that the signal pep...

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Detalhes bibliográficos
Publicado no:Microb Cell Fact
Main Authors: Mirzadeh, Kiavash, Shilling, Patrick J., Elfageih, Rageia, Cumming, Alister J., Cui, Huanhuan L., Rennig, Maja, Nørholm, Morten H. H., Daley, Daniel O.
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2020
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7137448/
https://ncbi.nlm.nih.gov/pubmed/32264894
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-020-01339-8
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