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Synthesis of long cDNA from viral RNA template()

Methods to make long and reliable cDNA from viral RNA template have been optimized. The conditions of the denaturation of the viral RNA template were most critical. For synthesis of the first DNA strand, the concentration of the primer and the presence of an RNase inhibitor were important. During th...

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Bibliografische gegevens
Gepubliceerd in:Gene Anal Tech
Hoofdauteurs: Lenstra, J.A., De Groot, R.J., Jacobs, L., Kusters, J.G., Niesters, H.G.M., Van Der Zeijst, B.A.M.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Published by Elsevier B.V. 1988
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7135641/
https://ncbi.nlm.nih.gov/pubmed/3192156
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/0735-0651(88)90017-9
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