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Synthesis of long cDNA from viral RNA template()

Methods to make long and reliable cDNA from viral RNA template have been optimized. The conditions of the denaturation of the viral RNA template were most critical. For synthesis of the first DNA strand, the concentration of the primer and the presence of an RNase inhibitor were important. During th...

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Detalhes bibliográficos
Publicado no:Gene Anal Tech
Main Authors: Lenstra, J.A., De Groot, R.J., Jacobs, L., Kusters, J.G., Niesters, H.G.M., Van Der Zeijst, B.A.M.
Formato: Artigo
Idioma:Inglês
Publicado em: Published by Elsevier B.V. 1988
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7135641/
https://ncbi.nlm.nih.gov/pubmed/3192156
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/0735-0651(88)90017-9
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